LOCUS       sequence-163143-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-dependent dTomato expression in forebrain GABA-ergic
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-Flex-dTomato-Fishell_7
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..44
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    95..128
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(176..195)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          178..310
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gtggcgcgcc ataacttcgt ataatgtatg ctatacgaag ttattaagag gtttcatatt
       61 gctaatagca gctacaatcc agctaccatt ctgcataact tcgtataaag tatcctatac
      121 gaagttattc cggagtcgac ctttcttaag agctgtaatt gaactgggag tggacacctg
      181 tggagagaaa ggcaaagtgg atgtcagtaa gaccaatagg tgcctatcag aaacgcaaga
      241 gtcttctctg tctcgacaag cccagtttct attggtctcc ttaaacctgt cttgtaacct
      301 tgatacttac ctgcccagtg cctcacgacc aacttctgca gcttaaggcg ccgcgctctg
      361 cttctggaag gctgcgctcc tcgacctgca ggctagaagc aaatgtaagc aatagatggc
      421 tctgccctga cttttatgcc cagcccgggc tgcaggaatt cgatatcaag cttcctactg
      481 tgaaactttg ggttcgtagc tccccaggat caattctgaa caaagcctcc agctgcagtg
      541 ccatccaatt tgaagcagac attggggaca atttaaggtt tttatccaca agaaggtttt
      601 tttccattct cttaaatgca gccataatta gagtaatttt tcatgtagcc cgctgattac
      661 agcgttttta ccgtcaaaga taattacctg taattttctt ccacttttaa tactaaaaag
      721 ccatctttat ttagattcag gaacaggaaa ggcgaaacaa aagagggaaa ttattctgtt
      781 attcatacac aaattgcaga gacgtaggac ctaaaattga aaattaacca aaattataat
      841 gctgaaaaga atggaagagg ctgcagaagt acagctggta gtggggcttt gctgaattat
      901 tcaaattacg ttagggagaa tgctaccata catcgaaacc aacactaatt tttcgttaaa
      961 aaaa
//
