LOCUS       sequence-163145-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-dependent dTomato expression in forebrain GABA-ergic
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-Flex-dTomato-Fishell_7
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..143)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(194..227)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      247..256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(389..407)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     657..676
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     851..874
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attccctgca ggggtggggc gcgccatcga taagcttgat atcgaattca taacttcgta
      121 tagcatacat tatacgaagt tattctttgc accattctaa agaataacag tgataatttc
      181 tgggttaagg caaataactt cgtataggat actttatacg aagttatgct agcgccacca
      241 ctagtagcca ccatggccgt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
      301 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
      361 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      421 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
      481 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
      541 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
      601 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
      661 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
      721 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
      781 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
      841 gtggacacca agctggacat cacctcccac aacg
//
