LOCUS       sequence-163151-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This 
            plasmid does NOT contain Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: pLenti-sgCCR5
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     135..154
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        233..308
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    356..473
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        542..753
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(779..798)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
       61 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      121 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      181 ctttatatat cttgtggaaa ggacgaaaca ccgacaagtg tgatcacttg gggttttaga
      241 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      301 gtcggtgctt ttttgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
      361 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      421 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      481 attacaggga cagcagagat ccactttggc gccggctcga gtggctccgg tgcccgtcag
      541 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      601 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      661 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      721 ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gacgcgggat ccgccaccat
      781 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
      841 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
      901 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
      961 cggcaaggtg tgggtcgcgg acgacggcgc
//
