LOCUS       sequence-163159-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This 
            plasmid does NOT contain Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: pLenti-sgTPST2
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(45..293)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(103..122)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(273..293)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(471..515)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    700..933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 acttgctatt tctagctcta aaaccaggga tgtggccagt ccacggtgtt tcgtcctttc
       61 cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag
      121 tccattttaa aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt
      181 cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta
      241 acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctctctgggt
      301 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
      361 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
      421 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
      481 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
      541 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
      601 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
      661 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
      721 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
      781 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
      841 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
      901 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
      961 accactcttc tctttgcctt ggtgggtgct actcc
//
