LOCUS       sequence-163175-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses cytoplasmic copies of the Skp and Trigger Factor 
            protein folding chaperones..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pACYC-Skp-TF
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    494..518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(518..537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(519..537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        583..660
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(680..699)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tagcattatg cggccgcaag cttatttaac ctgtttcagt acgtcggcag tgatgtcttt
       61 tacatcgctg ctgttgtaag caacggcgtt tgcatcaaca accagatcga tatcctggct
      121 gttggcaacg gatttcacag cagtctggat acgagtaacc agtttgccgc gttcttcgtt
      181 ggaacgacgt gcgcgatcct gctcaaaagc ctgcgctttc tgagcaaaag tctggcgctg
      241 agccatcacg tctttttcca gcttagtgcg atcgctgccc gctttcatgg actgcagctt
      301 tttcatttta gcctgcagat cggtttccat acgctgcagt tcgctggcac ggcctttgaa
      361 ctcattttcc agcgtgttag aaacaccggt tttctgcgct acctgctgga acaggctgcc
      421 catgttgacg attgcaattt tgtcagccat ggtatatctc cttattaaag ttaaacaaaa
      481 ttatttctac aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      541 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
      601 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
      661 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
      721 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      781 gcgatggcgg agctgaatta cattcccaac cgcgtgg
//
