LOCUS       sequence-163178-        5703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NFKB1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NFKB1_WT
FEATURES             Location/Qualifiers
     source          1..5703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3103..3202)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3219..3238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3220..3238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3243..3259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3243..3259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3690..4481
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4574..5162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5063..5082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5316..5333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5492..5519)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5611..5697)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcagaa gatgatccat atttgggaag gcctgaacaa atgtttcatt
      241 tggatccttc tttgactcat acaatattta atccagaagt atttcaacca cagatggcac
      301 tgccaacagc agatggccca taccttcaaa tattagagca acctaaacag agaggatttc
      361 gtttccgtta tgtatgtgaa ggcccatccc atggtggact acctggtgcc tctagtgaaa
      421 agaacaagaa gtcttaccct caggtcaaaa tctgcaacta tgtgggacca gcaaaggtta
      481 ttgttcagtt ggtcacaaat ggaaaaaata tccacctgca tgcccacagc ctggtgggaa
      541 aacactgtga ggatgggatc tgcactgtaa ctgctggacc caaggacatg gtggtcggct
      601 tcgcaaacct gggtatactt catgtgacaa agaaaaaagt atttgaaaca ctggaagcac
      661 gaatgacaga ggcgtgtata aggggctata atcctggact cttggtgcac cctgaccttg
      721 cctatttgca agcagaaggt ggaggggacc ggcagctggg agatcgggaa aaagagctaa
      781 tccgccaagc agctctgcag cagaccaagg agatggacct cagcgtggtg cggctcatgt
      841 ttacagcttt tcttccggat agcactggca gcttcacaag gcgcctggaa cccgtggtat
      901 cagacgccat ctatgacagt aaagccccca atgcatccaa cttgaaaatt gtaagaatgg
      961 acaggacagc tggatgtgtg actggagggg aggaaattta tcttctttgt gacaaagttc
     1021 agaaagatga catccagatt cgattttatg aagaggaaga aaatggtgga gtctgggaag
     1081 gatttggaga tttttccccc acagatgttc atagacaatt tgccattgtc ttcaaaactc
     1141 caaagtataa agatattaat attacaaaac cagcctctgt gtttgtccag cttcggagga
     1201 aatctgactt ggaaactagt gaaccaaaac ctttcctcta ctatcctgaa atcaaagata
     1261 aagaagaagt gcagaggaaa cgtcagaagc tcatgcccaa tttttcggat agtttcggcg
     1321 gtggtagtgg tgccggagct ggaggcggag gcatgtttgg tagtggcggt ggaggagggg
     1381 gcactggaag tacaggtcca gggtatagct tcccacacta tggatttcct acttatggtg
     1441 ggattacttt ccatcctgga actactaaat ctaatgctgg gatgaagcat ggaaccatgg
     1501 acactgaatc taaaaaggac cctgaaggtt gtgacaaaag tgatgacaaa aacactgtaa
     1561 acctctttgg gaaagttatt gaaaccacag agcaagatca ggagcccagc gaggccaccg
     1621 ttgggaatgg tgaggtcact ctaacgtatg caacaggaac aaaagaagag agtgctggag
     1681 ttcaggataa cctctttcta gagaaggcta tgcagcttgc aaagaggcat gccaatgccc
     1741 ttttcgacta cgcggtgaca ggagacgtga agatgctgct ggccgtccag cgccatctca
     1801 ctgctgtgca ggatgagaat ggggacagtg tcttacactt agcaatcatc caccttcatt
     1861 ctcaacttgt gagggatcta ctagaagtca catctggttt gatttctgat gacattatca
     1921 acatgagaaa tgatctgtac cagacgccct tgcacttggc agtgatcact aagcaggaag
     1981 atgtggtgga ggatttgctg agggctgggg ccgacctgag ccttctggac cgcttgggta
     2041 actctgtttt gcacctagct gccaaagaag gacatgataa agttctcagt atcttactca
     2101 agcacaaaaa ggcagcacta cttcttgacc accccaacgg ggacggtctg aatgccattc
     2161 atctagccat gatgagcaat agcctgccat gtttgctgct gctggtggcc gctggggctg
     2221 acgtcaatgc tcaggagcag aagtccgggc gcacagcact gcacctggct gtggagcacg
     2281 acaacatctc attggcaggc tgcctgctcc tggagggtga tgcccatgtg gacagtacta
     2341 cctacgatgg aaccacaccc ctgcatatag cagctgggag agggtccacc aggctggcag
     2401 ctcttctcaa agcagcagga gcagatcccc tggtggagaa ctttgagcct ctctatgacc
     2461 tggatgactc ttgggaaaat gcaggagagg atgaaggagt tgtgcctgga accacgcctc
     2521 tagatatggc caccagctgg caggtatttg acatattaaa tgggaaacca tatgagccag
     2581 agtttacatc tgatgattta ctagcacaag gagacatgaa acagctggct gaagatgtga
     2641 agctgcagct gtataagtta ctagaaattc ctgatccaga caaaaactgg gctactctgg
     2701 cgcagaaatt aggtctgggg atacttaata atgccttccg gctgagtcct gctccttcca
     2761 aaacacttat ggacaactat gaggtctctg ggggtacagt cagagagctg gtggaggccc
     2821 tgagacaaat gggctacacc gaagcaattg aagtgatcca ggcagcctcc agcccagtga
     2881 agaccacctc tcaggcccac tcgctgcctc tctcgcctgc ctccacaagg cagcaaatag
     2941 acgagctccg agacagtgac agtgtctgcg acagcggcgt ggagacatcc ttccgcaaac
     3001 tcagctttac cgagtctctg accagtggtg cctcactgct aactctcaac aaaatgcccc
     3061 atgattatgg gcaggaagga cctctagaag gcaaaattta gttgccaact ttcttgtaca
     3121 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3181 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3241 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3301 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     3361 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3421 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3481 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3541 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3601 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3661 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3721 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3781 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3841 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3901 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3961 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     4021 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     4081 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4141 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4201 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4261 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4321 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4381 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4441 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4501 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4681 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5341 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5401 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5461 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5521 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5581 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5641 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5701 cct
//
