LOCUS       sequence-163182-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses a hisx6 tagged copy of the Ivy protein and the wild 
            type human lysozyme protein, both in the cytoplasmic compartment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRSF-Ivy-his-hLYZ
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             474..518
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(556..574)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      570..617
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac
       61 atatgaaggt ctttgaaagg tgtgagttgg ccagaactct gaaaagattg ggaatggatg
      121 gctacagggg aatcagccta gcaaactgga tgtgtttggc caaatgggag agtggttaca
      181 acacacgagc tacaaactac aatgctggag acagaagcac tgattatggg atatttcaga
      241 tcaatagccg ctactggtgt aatgatggca aaaccccagg agcagttaat gcctgtcatt
      301 tatcctgcag tgctttgctg caagataaca tcgctgatgc tgtagcttgt gcaaagaggg
      361 ttgtccgtga tccacaaggc attagagcat gggtggcatg gagaaatcgt tgtcaaaaca
      421 gagatgtccg tcagtatgtt caaggttgtg gagtgtaata actcgagtct ggtaaagaaa
      481 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagcgca gcttaattaa
      541 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      601 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
      661 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
      721 ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg gcacttttcg ggga
//
