LOCUS       sequence-163185-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing ALCAM-p2A-E2Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: ALCAM-p2A-E2Crimson
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..126
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(444..463)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
       61 gtacctagtg gaaccggaac ccttaaacat gtataacttc gtataatgta tgctatacga
      121 agttattagg tccctcgacg aatttcgacc actgtgctgg cggccggccg ctttacttgt
      181 acatcacagg aacaggtggt ggcgggcctc ggcgcgctcg tactgctcca ccacggtgta
      241 gtcctcgttg tgggaggtga tgtcgagctt gtagtccacg tagtggtagc cgggcagctt
      301 cacgggcttc ttggccatgt agatggactt gaactcacac aggtagtggc cgccgccctt
      361 cagcttcagc gccatgtggt tctcgccctt cagcacgccg tcgcgggggt agttgcgctc
      421 agtggagggc tcccagccca gagtcttctt ctgcattacg gggccgtcgg aggggaagtt
      481 cacgccgatg aacttcacgt ggtagatgag ggtgccgtcc tgcagggagg agtcctgggt
      541 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
      601 gaaggactgc ttgaggtagt cggggatgtc ggcggggtgc ttgatgtacg ccttggagcc
      661 gtagaagaac tggggggaca ggatgtccca ggcgaagggc agggggccgc ccttggtcac
      721 ttgcagcttg gcggtctggg tgccctcgta gggcttgccc tcgcccacgc cctcgatctc
      781 ga
//
