LOCUS       sequence-163186-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein 
            folding chaperones..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pACYC-Skp-GroEL/ES
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..18
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             110..163
                     /codon_start=1
                     /product="GroES chaperone mobile loop that interacts with 
                     GroEL"
                     /label=S loop
                     /translation="EVETKSAGGIVLTGSAAA"
ORIGIN
        1 attgtgagcg gataacaatt ccccatctta gtatattagt taagtataag aaggagatat
       61 acatatgaat attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag aagttgaaac
      121 taaatctgct ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca cccgcggcga
      181 agtgctggct gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc cgctggatgt
      241 gaaagttggc gacatcgtta ttttcaacga tggctacggt gtgaaatctg agaagatcga
      301 caatgaagaa gtgttgatca tgtccgaaag cgacattctg gcaattgttg aagcgtaatc
      361 cgcgcacgac actgaacata cgaatttaag gaataaagat aatggcagct aaagacgtaa
      421 aattcggtaa cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg gcagatgcag
      481 tgaaagttac cctcggtcca aaaggccgta acgtagttct ggataaatct ttcggtgcac
      541 cgaccatcac caaagatggt gtttccgttg ctcgtgaaat cgaactggaa gacaagttcg
      601 aaaatatggg tgcgcagatg gtgaaagaag ttgcctctaa agcaaacgac gctgcaggcg
      661 acggtaccac cactgcaacc gtactggctc aggctatcat cactgaaggt ctgaaagctg
      721 ttgctgcggg catgaacccg atggacctga aacgtggtat cgacaaagcg gttaccgctg
      781 cagttgaaga actgaa
//
