LOCUS       sequence-163188-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing RAP2A-p2A-E2Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: RAP2A-p2A-E2Crimson
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..125
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(183..854)
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="STENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAKLQ
                     VTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHMAL
                     KLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHHLF
                     L"
     primer_bind     complement(443..462)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(858..914)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 caatctttca caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattg
       61 tacctagtgg aaccggaacc cttaaacatg tataacttcg tataatgtat gctatacgaa
      121 gttattaggt ccctcgacga atttcgacca ctgtgctggc ggccggccgc tttacttgta
      181 catcacagga acaggtggtg gcgggcctcg gcgcgctcgt actgctccac cacggtgtag
      241 tcctcgttgt gggaggtgat gtcgagcttg tagtccacgt agtggtagcc gggcagcttc
      301 acgggcttct tggccatgta gatggacttg aactcacaca ggtagtggcc gccgcccttc
      361 agcttcagcg ccatgtggtt ctcgcccttc agcacgccgt cgcgggggta gttgcgctca
      421 gtggagggct cccagcccag agtcttcttc tgcattacgg ggccgtcgga ggggaagttc
      481 acgccgatga acttcacgtg gtagatgagg gtgccgtcct gcagggagga gtcctgggtc
      541 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
      601 aaggactgct tgaggtagtc ggggatgtcg gcggggtgct tgatgtacgc cttggagccg
      661 tagaagaact ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcact
      721 tgcagcttgg cggtctgggt gccctcgtag ggcttgccct cgcccacgcc ctcgatctcg
      781 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa gggcttgatg
      841 acgttctcag tgctattggg gccggggttc tcctccacgt cgccggcctg cttcagcagg
      901 ctgaaatttg tagctcctcc acctcctgcg gccgctgcgg ccgcttgtat g
//
