LOCUS       sequence-163192-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor 
            protein folding chaperones..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pACYC-GroEL/ES-TF
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
       61 gatatacata tgcaagtttc agttgaaacc actcaaggcc ttggccgccg tgtaacgatt
      121 actgtcgctg ctgacagcat cgagaccgct gttaaaagcg agctggtcaa cgttgcgaaa
      181 aaagtacgta ttgacggctt ccgcaaaggc aaagtgccaa tgaatatcgt tgctcagcgt
      241 tatggcgcgt ctgtacgcca ggacgttctg ggtgacctga tgagccgtaa cttcattgac
      301 gccatcatta aagaaaaaat caatccggct ggcgcaccga cttatgttcc gggcgaatac
      361 aagctgggtg aagacttcac ttactctgta gagtttgaag tttatccgga agttgaactg
      421 cagggtctgg aagcgatcga agttgaaaaa ccgatcgttg aagtgaccga cgctgacgtt
      481 gacggcatgc tggatactct gcgtaaacag caggcgacct ggaaagaaaa agacggcgct
      541 gttgaagcag aagaccgcgt aaccatcgac ttcaccggtt ctgtagacgg cgaagagttc
      601 gaaggcggta aagcgtctga tttcgtactg gcgatgggcc agggtcgtat gatcccgggc
      661 tttgaagacg gtatcaaagg ccacaaagct ggcgaagagt tcaccatcga cgtgaccttc
      721 ccggaagaat accacgcaga aagcctgaaa ggtaaagcag cgaaattcgc tatcaacctg
      781 aagaaagttg aagagcgtga a
//
