LOCUS       sequence-163198-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses cytoplasmic copies of the Skp protein folding chaperone
            and wild type human lysozyme...
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pACYC-Skp-hLYZ
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..20
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             478..522
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(560..578)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      574..621
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggattgtgag cggataacaa ttccccatct tagtatatta gttaagtata agaaggagat
       61 atacatatga aggtctttga aaggtgtgag ttggccagaa ctctgaaaag attgggaatg
      121 gatggctaca ggggaatcag cctagcaaac tggatgtgtt tggccaaatg ggagagtggt
      181 tacaacacac gagctacaaa ctacaatgct ggagacagaa gcactgatta tgggatattt
      241 cagatcaata gccgctactg gtgtaatgat ggcaaaaccc caggagcagt taatgcctgt
      301 catttatcct gcagtgcttt gctgcaagat aacatcgctg atgctgtagc ttgtgcaaag
      361 agggttgtcc gtgatccaca aggcattaga gcatgggtgg catggagaaa tcgttgtcaa
      421 aacagagatg tccgtcagta tgttcaaggt tgtggagtgt aataactcga gtctggtaaa
      481 gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag cgcagcttaa
      541 ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      601 cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg
      661 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
      721 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
      781 tatcact
//
