LOCUS       sequence-163214-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLentiCRISPRv1-sgSHMT1-G3
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac gatgacgata agatggcccc
       61 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat
      121 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      181 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      241 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      301 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      361 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      421 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      481 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
      541 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
      601 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
      661 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
      721 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
      781 ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg
      841 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
      901 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
      961 cgacctgttt ctggccgcca agaacctgtc cgacgcc
//
