LOCUS       sequence-163216-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ATR minigene to test splicing event.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Ilagan JO, Ramakrishnan A, Hayes B, Murphy ME, Zebari AS, Bradley P,
            Bradley RK
  TITLE     U2AF1 mutations alter splice site recognition in hematological 
            malignancies.
  JOURNAL   Genome Res. 2015 Jan;25(1):14-26. doi: 10.1101/gr.181016.114. Epub 
            2014 Sep 29.
  PUBMED    25267526
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.181016.114"; journalName: "Genome Res"; date: "2015-01";
            volume: "25"; issue: "1"; pages: "14-26"
COMMENT     Sequence Label: pUB6_ATR
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(43..60)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(70..111)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 actagaaggc acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg
       61 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaggg
      121 ccctctagac tcgagcggcc gccactgtgc tggattcaca tatatggagt ccaaccaaga
      181 tacatctggc atagtaagtt ttcatcagta gcttcttgta taaggtaatg cacatgtcct
      241 tcaatagata acggcagtcc tgtcactcta tttcgagtct tgattacacc ttgtagtcgc
      301 tgctcaatgt caagaacatg ggtcttggcc taaaaagaag aaacataaaa ccaaaaacag
      361 atgttaataa tttggaatta ctacctttct ttatattttg acatttaata aatgttaata
      421 ccattttact gaccaatacc ccttttgtaa gagcaaatac aggaaaagaa gaaattaaac
      481 aaatacagtt agcttgtgat actgatttgg aggctcgcaa gattgattca acatcaactg
      541 cttggacatg tttctggact acgtgaaata ccagggaatt atttacacat tacaattcta
      601 gaaaaataat tttttaaatc tgctttggaa gtgtttagga cttaaacatt tcaatgaata
      661 ttcaaataac aatgctgtac tgcctaatat ttacattatg ttagtacatg caaatgggtg
      721 ttctaagtga gctacttgga actgtacaat ttcttaagca tatctgaatg caaaaggaag
      781 aataacgtta ttagaagaat aattctcaga ggtggtgctg g
//
