LOCUS       sequence-163227-       12613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR, Goodman RH
  TITLE     Phosphorylated CREB binds specifically to the nuclear protein CBP.
  JOURNAL   Nature. 1993 Oct 28. 365(6449):855-9.
  PUBMED    8413673
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 Oct 28. 365(6449):855-9."
COMMENT     Sequence Label: pRc/RSV-m CBP-HA
FEATURES             Location/Qualifiers
     source          1..12613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        340..601
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      5513..5522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7988..8014
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8058..8075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8064..8288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8334..8847
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8421..8440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8631..8652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8870..8890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8875..9204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9055..9190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9271..10065
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9325..9344)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9935..9954
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10239..10373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10276..10295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10330..10349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10404..10420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10404..10420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10417..10439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10428..10444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10452..10458,10459..10476,10477..10482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10497..10518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10687..10704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10858..11446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10938..10957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11617..12408,12409..12477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12240..12259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12478..12582)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag
      241 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
      301 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaatact
      361 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
      421 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
      481 acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agatattgta
      541 tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat tggtgtgcac
      601 ctccaagctt gatatcgaat tcctgcagcc cgggggatcc agaatggccg agaacttgct
      661 ggacggaccg cccaacccca aacgagccaa actcagctcg cccggcttct ccgcgaatga
      721 caacacagat tttggatcat tgtttgactt ggaaaatgac cttcctgatg agctgatccc
      781 caatggagaa ttaagccttt taaacagtgg gaaccttgtt ccagatgctg cgtccaaaca
      841 taaacaactg tcagagcttc ttagaggagg cagcggctct agcatcaacc cagggatagg
      901 caatgtgagt gccagcagcc ctgtgcaaca gggccttggt ggccaggctc aggggcagcc
      961 gaacagtaca aacatggcca gcttaggtgc catgggcaag agccctctga accaaggaga
     1021 ctcatcaaca cccaacctgc ccaaacaggc agccagcacc tctgggccca ctccccctgc
     1081 ctcccaagca ctgaatccac aagcacaaaa gcaagtaggg ctggtgacca gtagtcctgc
     1141 cacatcacag actggacctg ggatctgcat gaatgctaac ttcaaccaga cccacccagg
     1201 ccttctcaat agtaactctg gccatagctt aatgaatcag gctcaacaag ggcaagctca
     1261 agtcatgaat ggatctcttg gggctgctgg aagaggaagg ggagctggaa tgccctaccc
     1321 tgctccagcc atgcaggggg ccacaagcag tgtgctggcg gagaccttga cacaggtttc
     1381 cccacaaatg gctggccatg ctggactaaa tacagcacag gcaggaggca tgaccaagat
     1441 gggaatgact ggtaccacaa gtccatttgg acaacccttt agtcaaactg gagggcagca
     1501 gatgggagcc actggagtta acccccagtt agccagcaaa cagagcatgg tcaatagttt
     1561 acctgctttt cctacagata tcaagaatac ttcagtcacc actgtgccaa atatgtccca
     1621 gttgcaaaca tcagtgggaa ttgtacccac acaagcaatt gcaacaggcc ccacagcaga
     1681 ccctgaaaaa cgcaaactga tacagcagca gctggttcta ctgcttcatg cccacaaatg
     1741 tcagagacga gagcaagcaa atggagaggt tcgagcctgt tctctcccac actgtcgaac
     1801 catgaaaaac gttttgaatc acatgacaca ttgtcaggct gggaaagcct gccaagttgc
     1861 ccattgtgca tcttcacgac aaatcatctc tcattggaag aactgcacac gacatgactg
     1921 tcctgtttgc ctccctttga aaaatgccag tgacaagcga aaccaacaaa ccatcctggg
     1981 atctccagct agtggaattc aaaacacaat tggttctgtt ggtgcagggc aacagaatgc
     2041 cacttcctta agtaacccaa atcccataga ccccagttcc atgcagcggg cctatgctgc
     2101 tctaggactc ccctacatga accagcctca gacgcagctg cagcctcagg ttcctggcca
     2161 gcaaccagca cagcctccag cccaccagca gatgaggact ctcaatgccc taggaaacaa
     2221 ccccatgagt atcccagcag gaggaataac aacagatcaa cagccaccaa acttgatttc
     2281 agaatcagct cttccaactt ccttgggggc taccaatcca ctgatgaatg atggttcaaa
     2341 ctctggtaac attggaagcc tcagcacgat acctacagca gcgcctcctt ccagcactgg
     2401 tgttcgaaaa ggctggcatg aacatgtgac tcaggaccta cggagtcatc tagtccataa
     2461 actcgttcaa gccatcttcc caactccaga ccctgcagct ctgaaagatc gccgcatgga
     2521 gaacctggtt gcctatgcta agaaagtgga gggagacatg tatgagtctg ctaatagcag
     2581 ggatgaatac tatcatttat tagcagagaa aatctataaa atacaaaaag aactagaaga
     2641 aaagcggagg tcacgtttac ataagcaagg catcctgggt aaccagccag ctttaccagc
     2701 ttctggggct cagccccctg tgattccacc agcccagtct gtaagacctc caaatgggcc
     2761 cctgcctttg ccagtgaatc gcatgcaggt ttctcaaggg atgaattcat ttaacccaat
     2821 gtccctggga aacgtccagt tgccacaggc acccatggga cctcgtgcag cctcccctat
     2881 gaaccactct gtgcagatga acagcatggc ctcagttccg ggtatggcca tttctccttc
     2941 acggatgcct cagcctccaa atatgatggg cactcatgcc aacaacatta tggcccaggc
     3001 acctactcag aaccagtttc tgccacagaa ccagtttcca tcatccagtg gggcaatgag
     3061 tgtgaacagt gtgggcatgg ggcaaccagc agcccaggca ggtgtttcac agggtcaggt
     3121 acctggagct gctctcccta accctctgaa catgctggca ccccaggcca gccagctgcc
     3181 ttgcccacca gtgacacagt caccattgca cccgactcca cctcctgctt ccacagctgc
     3241 tggcatgccc tctctccaac atccaacggc accaggaatg acccctcctc agccagcagc
     3301 tcccactcag ccatctactc ctgtgtcatc tgggcagact cctaccccaa ctcctggctc
     3361 agtgcccagc gctgcccaaa cacagagtac ccctacagtc caggcagcag cacaggctca
     3421 ggtgactcca cagcctcaga ccccagtgca gccaccatct gtggctactc ctcagtcatc
     3481 acagcagcaa ccaacgcctg tgcatactca gcctcctggc acaccgcttt ctcaggcagc
     3541 agccagcatt gataatagag tccctactcc ctcctctgtg accagtgctg aaaccagttc
     3601 ccagcagcca ggacccgatg tgcccatgct ggaaatgaag acagaggtgc agacagatga
     3661 tgctgagcct gaacctactg aatccaaggg ggaacctcgg tctgagatga tggaagagga
     3721 tttacaaggt tcttcccaag taaaagaaga gacagatacg acagagcaga agtcagagcc
     3781 aatggaagta gaagaaaaga aacctgaagt aaaagtggaa gctaaagagg aagaagagaa
     3841 cagttcgaac gacacagcct cacaatcaac atctccttcc cagccacgca aaaaaatctt
     3901 taaacccgag gagctacgcc aggcacttat gccaactcta gaagcactct atcgacagga
     3961 cccagagtct ttgccttttc gtcagcctgt agatcctcag ctcctaggaa tcccagatta
     4021 ttttgatata gtgaagaatc ctatggacct ttctaccatc aaacgaaagc tggacacagg
     4081 gcaatatcaa gaaccctggc agtatgtgga tgatgtctgg cttatgttca acaatgcgtg
     4141 gctatataat cgtaaaacgt cccgtgtata taaattttgc agtaaacttg cagaggtctt
     4201 tgaacaagaa attgaccctg tcatgcagtc tcttggatat tgctgtggac gaaagtatga
     4261 gttctcccca cagactttgt gctgttacgg aaagcagctg tgtacaattc ctcgtgatgc
     4321 agcctactac agctatcaga ataggtatca tttctgtgag aagtgtttca cagagatcca
     4381 gggcgagaat gtgaccctgg gtgacgaccc ttcccaacct cagacgacaa tttccaagga
     4441 tcaatttgaa aagaagaaaa atgatacctt agatcctgaa ccttttgttg actgcaaaga
     4501 gtgtggccgg aagatgcatc agatttgtgt tctacactat gacatcattt ggccttcagg
     4561 ttttgtgtgt gacaactgtt tgaagaaaac tggcagacct cggaaagaaa acaaattcag
     4621 tgctaagagg ctgcagacca cacgattggg aaaccactta gaagacagag tgaataagtt
     4681 tttgcggcgc cagaatcacc ctgaagctgg ggaggttttt gtcagagtgg tggccagctc
     4741 agacaagact gtggaggtca agccgggaat gaagtcaagg tttgtggatt ctggagagat
     4801 gtcggaatct ttcccatatc gtaccaaagc actctttgct tttgaggaga tcgatggagt
     4861 cgatgtgtgc ttttttggga tgcatgtgca agaatacggc tctgattgcc ccccaccaaa
     4921 tacaaggcgt gtatacatat cttatctgga cagtattcat ttcttccggc cccgctgcct
     4981 ccggacagct gtttaccatg agatcctcat cggatatctc gagtatgtga agaaattggg
     5041 gtatgtgaca ggacatattt gggcctgtcc cccaagtgaa ggagatgact atatctttca
     5101 ttgccacccc cctgaccaga aaatccccaa accaaaacga ctacaggagt ggtacaagaa
     5161 gatgctggac aaggcgtttg cagagaggat cattaacgac tataaggaca tcttcaaaca
     5221 agcgaacgaa gacaggctca cgagtgccaa ggagttgccc tattttgaag gagatttctg
     5281 gcctaatgtg ttggaagaaa gcattaagga actagaacaa gaagaagaag aaaggaaaaa
     5341 agaagagagt actgcagcga gtgagactcc tgagggcagt cagggtgaca gcaaaaatgc
     5401 gaagaaaaag aacaacaaga agaccaacaa aaacaaaagc agcattagcc gcgccaacaa
     5461 gaagaagccc agcatgccca atgtttccaa cgacctgtcg cagaagctgt atgccaccat
     5521 ggagaagcac aaggaggtat tctttgtgat tcatctgcat gctgggcctg ttatcagcac
     5581 tcagcccccc atcgtggacc ctgatcctct gcttagctgt gacctcatgg atgggcgaga
     5641 tgccttcctc accctggcca gagacaagca ctgggaattc tcttccttac gccgctccaa
     5701 atggtccact ctgtgcatgc tggtggagct gcacacacag ggccaggacc gctttgttta
     5761 tacctgcaat gagtgcaaac accatgtgga aacacgctgg cactgcactg tgtgtgagga
     5821 ctatgacctt tgtatcaatt gctacaacac aaagagccac acccataaga tggtgaagtg
     5881 ggggctaggc ctagatgatg agggcagcag tcagggtgag ccacagtcca agagccccca
     5941 ggaatcccgg cgtctcagca tccagcgctg catccagtcc ctggtgcatg cctgccagtg
     6001 tcgcaatgcc aactgctcac tgccgtcttg ccagaagatg aagcgagtcg tgcagcacac
     6061 caagggctgc aagcgcaaga ctaatggagg atgcccagtg tgcaagcagc tcattgctct
     6121 ttgctgctac cacgccaaac actgccaaga aaataaatgc cctgtgccct tctgcctcaa
     6181 catcaaacat aagctccgcc agcagcagat ccagcatcgc ctgcagcagg ctcagctcat
     6241 gcgccggcga atggcaacca tgaacacccg caatgtgcct cagcagagtt tgccttctcc
     6301 tacctcagca ccacccggga ctcctacaca gcagcccagc acaccccaaa caccacagcc
     6361 cccagcccag cctcagcctt cacctgttaa catgtcacca gctggcttcc ctaatgtagc
     6421 ccggactcag cccccaacaa tagtgtctgc tgggaagcct accaaccagg tgccagctcc
     6481 cccaccccct gcccagcccc cacctgcagc agtagaagca gcccggcaaa ttgaacgtga
     6541 ggcccagcag cagcagcacc tataccgagc aaacatcaac aatggcatgc ccccaggacg
     6601 tgcaggtatg gggaccccag gaagccaaat gactcctgtg ggcctgaatg tgccccgtcc
     6661 caaccaagtc agtgggcctg tcatgtctag tatgccacct gggcagtggc agcaggcacc
     6721 catccctcag cagcagccga tgccaggcat gcccaggcct gtaatgtcca tgcaggccca
     6781 ggcagcagtg gctgggccac ggatgcccaa tgtgcagcca ccaaggagca tctcgccaag
     6841 tgccctgcaa gacctgctac ggaccctaaa gtcacccagc tctcctcagc agcagcagca
     6901 ggtgctgaac atccttaaat caaacccaca gctaatggca gctttcatca aacagcgcac
     6961 agccaagtat gtggccaatc agcctggcat gcagccccag cccggacttc aatcccagcc
     7021 tggtatgcag ccccagcctg gcatgcacca gcagcctagt ttgcaaaacc tgaacgcaat
     7081 gcaagctggt gtgccacggc ctggtgtgcc tccaccacaa ccagcaatgg gaggcctgaa
     7141 tccccaggga caagctctga acatcatgaa cccaggacac aaccccaaca tgacaaacat
     7201 gaatccacag taccgagaaa tggtgaggag acagctgcta cagcaccagc agcagcagca
     7261 gcaacagcag cagcagcagc agcaacaaca aaatagtgcc agcttggccg ggggcatggc
     7321 gggacacagc cagttccagc agccacaagg acctggaggt tatgccccag ccatgcagca
     7381 gcaacgcatg caacagcacc tccccatcca gggcagctcc atgggccaga tggctgctcc
     7441 aatgggacaa cttggccaga tggggcagcc tgggctaggg gcagacagca cccctaatat
     7501 ccagcaggcc ctgcagcaac ggattctgca gcagcagcag atgaagcaac aaattgggtc
     7561 accaggccag ccgaacccca tgagccccca gcagcacatg ctctcaggac agccacaggc
     7621 ctcacatctc cctggccagc agatcgccac atcccttagt aaccaggtgc gatctccagc
     7681 ccctgtgcag tctccacggc cccaatccca acctccacat tccagcccgt caccacggat
     7741 acaaccccag ccttcaccac accatgtttc accccagact ggttcccctc accctggact
     7801 cgcagtcacc atggccagct ccatggatca gggacacctg gggaaccctg aacagagtgc
     7861 aatgctcccc cagctgaata cccccaacag gagcgcactg tccagtgaac tgtccctggt
     7921 tggtgatacc acgggagaca cactagaaaa gtttgtggag ggtttgaagc tggatccggg
     7981 gatggcctac ccatacgacg tgcctgacta cgcctcctag cggccgctcg agcatgcatc
     8041 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     8101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     8281 ctctatggct tctgaggcgg aaagaaccag ctggggctcg aggggggatc cccacgcgcc
     8341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8761 ttttaacaaa atattaacgc ttacaattta aatatttgct tatacaatct tcctgttttt
     8821 ggggcttttc tgattatcaa ccggggtggg taccgagctc gaattctgtg gaatgtgtgt
     8881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9181 tttggaggcc taggcttttg caaaaagctc ccgggagctt ggatatccat tttcggatct
     9241 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     9301 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     9361 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     9421 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     9481 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     9541 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     9601 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     9661 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     9721 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     9781 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     9841 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     9901 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     9961 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    10021 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
    10081 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
    10141 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    10201 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    10261 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10321 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtcgac
    10381 ctcgagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10621 cgctcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12601 gccacctgac gtc
//
