LOCUS       sequence-163232-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hU6 expression of gRNA NT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHu6-gRNA-NT1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(42..290)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(100..119)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(270..290)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        391..495
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(714..733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgctatttct agctctaaaa ccaattttcc caaacagagg tggtgtttcg tcctttccac
       61 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
      121 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
      181 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca
      241 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg
      301 accttggatc cagtcgactg aattggttcc tttaaagcct gcttttttgt acagctagcg
      361 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      781 tctcagaatg acttggttga gtactcacca gtcacagaa
//
