LOCUS       sequence-163235-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains two target sites consisting of PAM-NT1-6bp-gix psuedo 
            site-6bp-NT1-PAM flanking a kan/pA as previously described in the 
            pCALNL-eGFP plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pCALNL-eGFP-NT1-6-gix-6-NT1
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(206..225)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(287..309)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
       61 agatcaaatc cgactcgact ccccaatttt cccaaacaga ggtgctaagt ccaaaacctc
      121 ggtttacaga tttcgacctc tgtttgggaa aattggggac gcgttcgctc gaattaatca
      181 attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga
      241 aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga
      301 agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc
      361 gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca
      421 tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc
      481 gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc
      541 acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc
      601 gggggccgcc gcgcc
//
