LOCUS       sequence-163245-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV promoter driving expression of GinB catalytic domain fused with 
            linker to dCas9 in the order shown. Has a FLAG and NLS tag on the 
            c-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pGinB-8X(GGS)-dCas9-FLAG-NLS
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
       61 cactataggg agagccgcca ccatgctcat tggctacgtg cgcgtctcaa ctaacgacca
      121 gaataccgat cttcagagga acgcactggt ttgtgcaggc tgcgaacaga ttttcgagga
      181 caaactcagc gggacacgga cggacagacc tggcctcaag cgagcactca agaggctgca
      241 gaaaggagac actctggtgg tctggaaatt ggaccgcctg ggtcgaagca tgaagcatct
      301 catttctctg gttggcgaac tgcgagaaag ggggatcaac tttcgaagtc tgacggattc
      361 catagataca agcagcccca tgggccggtt cttcttctac gtgatgggtg cactggctga
      421 aatggaaaga gaactcatta tagagcgaac catggcaggg cttgcggctg ccaggaataa
      481 aggcaggcgg tttggaagac caccaaaggg tggatccgga gggtccggag gtagtggcgg
      541 cagcggtggt tcaggtggca gcggagggtc aggaggctct gataaaaagt attctattgg
      601 tttagctatc ggcactaatt ccgttggatg ggctgtcata accgatgaat acaaagtacc
      661 ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat tcgattaaaa agaatcttat
      721 cggtgccctc ctattcgata gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc
      781 tcggagaagg tatacacgtc gcaaga
//
