LOCUS       sequence-163247-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV promoter driving expression of GinB catalytic domain fused with 
            linker to dCas9 in the order shown. Has a FLAG and NLS tag on the 
            c-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pGinB-8X(GGS)-dCas9-FLAG-NLS
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..48)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(58..81)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggtgatgg tgatgatgac cggttcagac cttccttttc ttctttgggg aacctccctt
       61 gtcgtcatca tccttataat cggagccacc gtcaccccca agctgtgaca aatctatccg
      121 agtttcatat aatcccgtga tggattggtg aatcagtgtc gcgtctagca cctccttggt
      181 agaagtgtat cgtttgcgat ctatcgttgt gtcaaaatac ttgaatgcgg ctggagcgcc
      241 gaggttggta agagtaaaca aatggataat attttccgcc tgctcacgta tgggtttatc
      301 cctgtgcttg ttgtatgcgc ttaatacttt gtccagattg gcatcagcta ggatgactct
      361 cttactgaat tccgaaattt gctctatgat ttcgtcgaga taatgtttgt gctgctcaac
      421 aaaaagttgc ttctgttcgt tatcttcagg tgaacctttc aacttctcgt aatgggacgc
      481 taaatacagg aaattcacgt atttagacgg tagtgcgagt tcgttcccct tttgaagctc
      541 tccggcgcta gccaacatcc gttttcggcc attttctaac tcaaacagac tatactttgg
      601 tagtttaatt atgagatcct tttttacttc cttgtaacct ttcgcctcaa ggaagtcgat
      661 ggggttcttt tcaaaagacg agcgctccat aatcgttatc cccaataatt ctttgactga
      721 cttcagtttc ttggattttc ccttctcaac ttttgccact actaggacag aataggcaac
      781 tgtagg
//
