LOCUS       sequence-163249-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV promoter driving expression of GinB catalytic domain fused with 
            linker to dCas9 in the order shown. Has a FLAG and NLS tag on the 
            c-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pGinB-8X(GGS)-dCas9-FLAG-NLS
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt
       61 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
      121 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
      181 gacataaacc gtttatctga ttacgacgtc gatgccattg taccccaatc ctttttgaag
      241 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
      301 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
      361 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
      421 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca
      481 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag
      541 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
      601 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
      661 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt
      721 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag
      781 ataggcaagg ctacagccaa atacttcttt
//
