LOCUS       sequence-163252-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs 
            from the cas9 site and 5' of gix site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_gRNA_Ch10-rev2
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..294)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(104..123)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(274..294)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        395..499
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(718..737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acttgctatt tctagctcta aaactcccat cacaggccct gaggcggtgt ttcgtccttt
       61 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
      121 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
      181 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
      241 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttcctg
      301 cccgaccttg gatccagtcg actgaattgg ttcctttaaa gcctgctttt ttgtacagct
      361 agcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      781 ctat
//
