LOCUS       sequence-163254-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs 
            from the cas9 site and 5' of gix site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_gRNA_Ch10-rev1
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(41..289)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(99..118)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(269..289)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        390..494
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctatttctag ctctaaaacc tcccatcaca ggccctgagc ggtgtttcgt cctttccaca
       61 agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca
      121 ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg
      181 tattttgtac taatatcttt gtgtttacag tcaaattaat tctaattatc tctctaacag
      241 ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct tcctgcccga
      301 ccttggatcc agtcgactga attggttcct ttaaagcctg cttttttgta cagctagcga
      361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      661 agagttttcg ccccgaagaa cgtt
//
