LOCUS       sequence-163256-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs 
            from the cas9 site and 3' of gix site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_gRNA_Ch10-for2
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(44..292)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(102..121)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(272..292)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        393..497
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(716..735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgctattt ctagctctaa aacgaggaga agggtcatgg gccggtgttt cgtcctttcc
       61 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      121 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      181 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
      241 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttcctgcc
      301 cgaccttgga tccagtcgac tgaattggtt cctttaaagc ctgctttttt gtacagctag
      361 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      421 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      481 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      541 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      601 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      661 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      721 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      781 attctcaga
//
