LOCUS       sequence-163260-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCALNL reporter contains two target sites consisting of PAM Cas9 
            site-gix psuedo site-Cas9 site-PAM that match region in centromeres 
            of chromosomes 5 (numbers in common name refer to pos. in hg38).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pCALNL_Ch5_155183064-155183141
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(204..223)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(285..307)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
       61 atcaaatccg actcgactcc acagttaatc acatgagttc cggtctcaga taaacctagg
      121 tttggacctt agctctacct ctcatgagcc ggtgggacgc gttcgctcga attaatcaat
      181 tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa
      241 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag
      301 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga
      361 ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt
      421 tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc
      481 ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac
      541 gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg
      601 gggccgccgc gcccc
//
