LOCUS       sequence-163262-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCALNL reporter contains two target sites consisting of PAM Cas9 
            site-gix psuedo site-Cas9 site-PAM that match region in centromeres 
            of chromosomes 12 (numbers in common name refer to pos. in hg38).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pCALNL_Ch12_62418577-62418652
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    190..324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(214..233)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     268..287
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(615..634)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
       61 actgcagaat tcaatttaaa tcgtaccgag cgactcgagc cattccattc attgtgctat
      121 ttgttgcctg taaaccttgg ttttctacct ttactttttg ggggagaggg agtgggtgcg
      181 ttcggatttg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      301 attataagct gcaataaaca agttatcgat ggggtgggcg aagaactcca gcatgagatc
      361 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
      421 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
      481 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
      541 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
      601 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
      661 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
      721 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
//
