LOCUS       sequence-163263-         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCALNL reporter contains two target sites consisting of PAM Cas9 
            site-gix psuedo site-Cas9 site-PAM that match region in centromeres 
            of chromosomes 12 (numbers in common name refer to pos. in hg38).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pCALNL_Ch12_62418577-62418652
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(202..221)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(283..305)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
       61 atcaaatccg actcgactcc attccattca ttgtgctatt tgttgcctgt aaaccttggt
      121 tttctacctt tactttttgg gggagaggga gtggacgcgt tcgctcgaat taatcaattc
      181 tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa
      241 gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc
      301 gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg
      361 ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc
      421 cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct
      481 cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga
      541 ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg
      601 gccgc
//
