LOCUS       sequence-163267-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCALNL reporter contains two target sites consisting of PAM Cas9 
            site-gix psuedo site-Cas9 site-PAM that match region in centromeres 
            of chromosomes 13 (numbers in common name refers to pos. in hg38).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pCALNL_chr13_102010574-102010650
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(203..222)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(284..306)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
       61 atcaaatccg actcgactcc aggttttgga cttaagtctc ctcaacttta aaacctaggt
      121 ttttccagaa actcagctat gatcacttcc ctaggacgcg ttcgctcgaa ttaatcaatt
      181 ctttgccaaa atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa
      241 agaagaaggc atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc
      301 cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc cccccgcgga cagccccgag
      361 gctggagagg gagaagggga cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt
      421 ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc
      481 tcccagattt cggctccgca cagatttggg acaaaggaag tccctgcgcc ctctcgcacg
      541 attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg
      601 ggccgccgcg cccctccccc gagccctccc cg
//
