LOCUS       sequence-163272-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of 
            pCALNL_Ch5 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_Ch5_155183064-gRNA-rev
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(47..295)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(105..124)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(275..295)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        396..500
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacttgctat ttctagctct aaaacaccgg ctcatgagag gtagacggtg tttcgtcctt
       61 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
      121 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
      181 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
      241 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcttcct
      301 gcccgacctt ggatccagtc gaatgaattg gttcctttaa agcctgcttt tttgtacagc
      361 tagcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccatatt tgtttatttt
      421 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      481 aatattgaaa aaggaagagt atgagggaag cggtgatcgc cgaagtatcg actcaactat
      541 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
      601 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
      661 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
      721 cggcttcc
//
