LOCUS       sequence-163274-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of 
            pCALNL_Ch12 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_Ch12_62418577-gRNA-for
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..294)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(104..123)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(274..294)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        395..499
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acttgctatt tctagctcta aaacttccat tcattgtgct atttcggtgt ttcgtccttt
       61 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
      121 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
      181 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
      241 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttcctg
      301 cccgaccttg gatccagtcg aatgaattgg ttcctttaaa gcctgctttt ttgtacagct
      361 agcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccatattt gtttattttt
      421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      481 atattgaaaa aggaagagta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
      541 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
      601 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
      661 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
      721 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcac
//
