LOCUS       sequence-163279-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of 
            pCALNL_Ch13 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_Ch13_102010574-gRNA-rev
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(45..293)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(103..122)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(273..293)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        394..498
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cttgctattt ctagctctaa aacagggaag tgatcatagc tgacggtgtt tcgtcctttc
       61 cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag
      121 tccattttaa aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt
      181 cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta
      241 acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcttcctgc
      301 ccgaccttgg atccagtcga atgaattggt tcctttaaag cctgcttttt tgtacagcta
      361 gcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccatatttg tttatttttc
      421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      481 tattgaaaaa ggaagagtat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
      541 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      601 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
      661 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      721 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      781 gacatcattc cgtggcgtta tccagctaag cgcga
//
