LOCUS       sequence-163284-        5111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gli2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5111)
  TITLE     Fernandez-Zapico lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hGli2 flag3x
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..55
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     CDS             4875..4940
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccggacacaa ttgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
       61 agtgaaccgt cagaattaag cttgcggccg cgaattcaat ggagacgtct gcctcagcca
      121 ctgcctccga gaagcaagaa gccaaaagtg ggatcctgga ggccgctggc ttccccgacc
      181 cgggtaaaaa ggcctctcct ttggtggtgg ctgcagcggc agcagcagcg gtagctgccc
      241 aaggagtgcc gcagcatctc ttgccaccat tccatgcgcc cctaccgatt gacatgcgac
      301 accaggaagg aaggtaccat tacgagcctc attctgtcca cggtgtgcac gggccccctg
      361 ccctcagcgg cagccctgtc atctctgaca tctccttgat ccggctttcc ccgcacccgg
      421 ctggccctgg ggagtccccc ttcaacgccc cccacccgta cgtgaacccc cacatggagc
      481 actacctccg ttctgtgcac agcagcccca cgctctccat gatctctgca gccaggggcc
      541 tcagccccgc tgatgtggcc caggagcacc ttaaggagag gggactgttt ggccttcctg
      601 ctccaggcac caccccctca gactattacc accagatgac cctcgtggca ggccaccccg
      661 cgccctacgg ggacctgctg atgcagagcg ggggcgctgc cagcgcaccc catctccacg
      721 actacctcaa ccccgtggac gtgtcccgtt tctccagccc gcgggtgacg ccccgcctga
      781 gccgcaagcg ggcgctgtcc atctccccac tctcagacgc cagcctggac ctgcagcgga
      841 tgatccgcac ctcacccaac tcgctagtgg cctacatcaa caactcccga agcagctcag
      901 cggccagcgg ttcctacggg catctgtcag cgggtgccct cagcccagcc ttcaccttcc
      961 cccaccccat caaccccgtg gcctaccagc agattctgag ccagcagagg ggtctggggt
     1021 cagcctttgg acacacacca cccctgatcc agccctcacc caccttcctg gcccagcagc
     1081 ccatggccct cacctccatc aatgccacgc ccacccagct cagcagcagc agcaactgtc
     1141 tgagtgacac caaccagaac aagcagagca gtgagtcggc cgtcagcagc accgtcaacc
     1201 ctgtcgccat tcacaagcgc agcaaggtca agaccgagcc tgagggcctg cggccggcct
     1261 cccctctggc gctgacgcag ggccaggtgt ctggacacgg ctcatgtggg tgtgcccttc
     1321 ccctctccca ggagcagctg gctgacctca aggaagatct ggacagggat gactgtaagc
     1381 aggaggctga ggtggtcatc tatgagacca actgccactg ggaagactgc accaaggagt
     1441 acgacaccca ggagcagctg gtgcatcaca tcaacaacga gcacatccac ggggagaaga
     1501 aggagtttgt gtgccgctgg caggcctgca cgcgggagca gaagcccttc aaggcgcagt
     1561 acatgctggt ggtgcacatg cggcgacaca cgggcgagaa gccccacaag tgcacgttcg
     1621 agggctgctc gaaggcctac tcccgcctgg agaacctgaa gacacacctg cggtcccaca
     1681 ccggggagaa gccatatgtg tgtgagcacg agggctgcaa caaagccttc tccaacgcct
     1741 cggaccgcgc caagcaccag aatcgcaccc actccaacga gaaaccctac atctgcaaga
     1801 tcccaggctg caccaagaga tacacagacc ccagctctct ccggaagcat gtgaaaacgg
     1861 tccacggccc agatgcccac gtcaccaaga agcagcgcaa tgacgtgcac ctccgcacac
     1921 cgctgctcaa agagaatggg gacagtgagg ccggcacgga gcctggcggc ccagagagca
     1981 ccgaggccag cagcaccagc caggccgtgg aggactgcct gcacgtcaga gccatcaaga
     2041 ccgagagctc cgggctgtgt cagtccagcc ccggggccca gtcgtcctgc agcagcgagc
     2101 cctctcctct gggcagtgcc cccaacaatg acagtggcgt ggagatgccg gggacggggc
     2161 ccgggagcct gggagacctg acggcactgg atgacacacc cccaggggcc gacacctcag
     2221 ccctggctgc cccctccgct ggtggcctcc agctgcgcaa acacatgacc accatgcacc
     2281 ggttcgagca gctcaagaag gagaagctca agtcactcaa ggattcctgc tcatgggccg
     2341 ggccgactcc acacacgcgg aacaccaagc tgcctcccct cccgggaagt ggctccatcc
     2401 tggaaaactt cagtggcagt gggggcggcg ggcccgcggg gctgctgccg aacccgcggc
     2461 tgtcggagct gtccgcgagc gaggtgacca tgctgagcca gctgcaggag cgccgcgaca
     2521 gctccaccag cacggtcagc tcggcctaca ccgtgagccg ccgctcctcc ggcatctccc
     2581 cctacttctc cagccgccgc tccagcgagg cctcgcccct gggcgccggc cgcccgcaca
     2641 acgcgagctc cgctgactcc tacgacccca tctccacgga cgcgtcgcgg cgctcgagcg
     2701 aggccagcca gtgcagcggc ggctccgggc tgctcaacct cacgccggcg cagcagtaca
     2761 gcctgcgggc caagtacgcg gcagccactg gcggcccccc gcccactccg ctgccgggcc
     2821 tggagcgcat gagcctgcgg accaggctgg cgctgctgga cgcgcccgag cgcacgctgc
     2881 ccgccggctg cccacgccca ctggggccgc ggcgtggcag cgacgggccg acctatggcc
     2941 acggccacgc gggggctgcg cccgccttcc cccacgaggc tccaggcggc ggagccaggc
     3001 gggccagcga ccctgtgcgg cggcccgatg ccctgtccct gccgcgggtg cagcgcttcc
     3061 acagcaccca caacgtgaac cccggcccgc tgccgccctg tgccgacagg cgaggcctcc
     3121 gcctgcagag ccacccgagc accgacggcg gcctggcccg cggcgcctac tcgccccggc
     3181 cgcctagcat cagcgagaac gtggcgatgg aggccgtggc ggcaggagtg gacggcgcgg
     3241 ggcccgaggc cgacctgggg ctgccggagg acgacctggt gcttccagac gacgtggtgc
     3301 agtacatcaa ggcgcacgcc agtggcgctc tggacgaggg caccgggcag gtgtatccca
     3361 cggaaagcac tggcttctct gacaacccca gactacccag cccggggctg cacggccagc
     3421 gcaggatggt ggctgcggac tccaacgtgg gcccctccgc ccctatgctg ggaggatgcc
     3481 agttaggctt tggggcgccc tccagcctga acaaaaataa catgcctgtg cagtggaatg
     3541 aggtgagctc cggcaccgta gacgccctgg ccagccaggt gaagcctcca ccctttcctc
     3601 agggcaacct ggcggtggtg cagcagaagc ctgcctttgg ccagtacccg ggctacagtc
     3661 cgcaaggcct acaggctagc cctgggggcc tggacagcac gcagccacac ctgcagcccc
     3721 gcagcggagc cccctcccag ggcatcccca gggtaaacta catgcagcag ctgcgacagc
     3781 cagtggcagg cagccagtgt cctggcatga ctaccactat gagcccccat gcctgctatg
     3841 gccaagtcca cccccagctg agccccagca ccatcagtgg ggccctcaac cagttccccc
     3901 aatcctgcag caacatgcca gccaagccag ggcatctggg gcaccctcag cagacagaag
     3961 tggcacctga ccccaccacg atgggcaatc gccacaggga acttggggtc cccgattcag
     4021 ccctggctgg agtgccacca cctcacccag tccagagcta cccacagcag agccatcacc
     4081 tggcagcctc catgagccag gagggctacc accaggtccc cagccttctg cctgcccgcc
     4141 agcctggctt catggagccc caaacaggcc cgatgggggt ggctacagca ggctttggcc
     4201 tagtgcagcc ccggcctccc ctcgagccca gccccactgg ccgccaccgt ggggtacgtg
     4261 ctgtgcagca gcagctggcc tacgccaggg ccacaggcca tgccatggct gccatgccgt
     4321 ccagtcagga aacagcagag gctgtgccca agggagcgat gggcaacatg gggtcggtgc
     4381 ctccccagcc gcctccgcag gacgcaggtg gggccccgga ccacagcatg ctctactact
     4441 acggccagat ccacatgtac gaacaggatg gaggcctgga gaacctcggg agctgccagg
     4501 tcatgcggtc ccagccacca cagccacagg cctgtcagga cagcatccag ccccagccct
     4561 tgccctcacc aggggtcaac caggtgtcca gcactgtgga ctcccagctc ctggaggccc
     4621 cccagattga cttcgatgcc atcatggatg atggcgatca ctcgagtttg ttctcgggtg
     4681 ctctgagccc cagcctcctc cacagcctct cccagaactc ctcccgcctc accacccccc
     4741 gaaactcctt gaccctgccc tccatccccg caggcatcag caacatggct gtcggggaca
     4801 tgagctccat gctcaccagc ctcgccgagg agagcaagtt cctgaacatg atgacctcta
     4861 gaggatcccg ggctgactac aaagaccatg acggtgatta taaagatcat gacatcgact
     4921 acaaggatga cgatgacaag tagtgatccc gggtggcatc cctgtgaccc ctccccagtg
     4981 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     5041 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     5101 gtatgtctag a
//
