LOCUS       sequence-163297-        3849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PRKACG , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PRKACG_WT
FEATURES             Location/Qualifiers
     source          1..3849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1249..1348)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1365..1384)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1366..1384)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1389..1405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1389..1405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1836..2627
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2720..3308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3209..3228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3462..3479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3638..3665)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3757..3843)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgggcaac gcccccgcca agaaggacac cgagcaggag gagagcgtga
      241 acgagttcct agccaaagcc agaggagatt tcctctacag atggggaaac cccgctcaaa
      301 acaccgccag ctcggatcag ttcgaacggc tcaggacgct gggcatgggc tccttcgggc
      361 gggtgatgct ggtgaggcac caggagaccg gcggccacta cgccatgaag atcctcaaca
      421 agcagaaggt ggtgaagatg aagcaggtcg agcacatact gaacgagaag cgcatcctgc
      481 aggcgatcga ctttccgttc ctcgtcaagc tccagttctc ctttaaggac aactcctacc
      541 tgtacctggt gatggagtac gtgccgggtg gggagatgtt ctcccgccta cagcgcgtcg
      601 gaaggtttag cgagccccat gcctgtttct atgccgccca ggtcgtcctg gccgtccagt
      661 acctacactc gctcgacctc atccaccgcg acctgaagcc cgagaatctc ctcatcgacc
      721 agcagggcta cctgcaggtg acggacttcg gtttcgccaa gcgcgtgaag ggccgcactt
      781 ggaccttgtg cgggacccca gagtacctgg cccccgagat catcctgagc aaaggctaca
      841 acaaggccgt ggactggtgg gccctagggg tgctcatcta tgagatggcc gtgggcttcc
      901 cacccttcta cgccgaccag cccatccaga tctacgagaa gatcgtctct gggagggtgc
      961 ggtttccctc caaactcagc tctgacctca agcatctgct gcggagcctg ctgcaggtgg
     1021 acctcaccaa gcgcttcgga aacctcagga acggggttgg cgacatcaag aaccacaagt
     1081 ggttcgccac aaccagctgg atcgccatct atgagaagaa ggtggaagct cccttcatcc
     1141 cgaagtacac aggccctggg gatgccagta actttgacga ctacgaggag gaagagctcc
     1201 ggatctccat caatgagaag tgtgccaagg agttttctga gttttagttg ccaactttct
     1261 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1321 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     1381 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1441 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     1501 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     1561 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1621 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1681 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     1741 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1801 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     1861 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1921 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1981 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2041 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2101 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2161 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2221 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2281 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2341 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2401 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2461 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2521 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2581 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     2641 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2701 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2761 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2821 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2881 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2941 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3061 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3301 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3361 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3421 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3481 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3601 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3661 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     3721 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     3781 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     3841 ttgatgcct
//
