LOCUS       sequence-163304-        4251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBPJ , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RBPJ_WT
FEATURES             Location/Qualifiers
     source          1..4251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3..21)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(26..42)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             473..1264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1357..1945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1846..1865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2099..2116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2275..2302)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2394..2480)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2529..2551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2543..2560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2544..2560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2576..2675
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4137..4236)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
       61 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
      121 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
      181 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
      241 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
      301 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
      361 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
      421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
      481 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      541 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      601 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      661 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      721 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
      781 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
      841 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
      901 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
      961 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1021 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1081 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1141 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     1201 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     1261 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     1321 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1381 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1441 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1501 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1561 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1621 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1681 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1741 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1801 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1861 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1921 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1981 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2041 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2101 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2161 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2221 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     2281 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     2341 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     2401 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     2461 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     2521 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
     2581 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
     2641 ttttataatg ccaactttgt acaaaaaagt tggcaccatg gggggctgca ggaaatttgg
     2701 tgagcggcct ccacctaaac gacttactag ggaagctatg cgaaattatt taaaagagcg
     2761 aggggatcaa acagtactta ttcttcatgc aaaagttgca cagaagtcat atggaaatga
     2821 aaaaaggttt ttttgcccac ctccttgtgt atatcttatg ggcagcggat ggaagaaaaa
     2881 aaaagaacaa atggaacgcg atggttgttc tgaacaagag tctcaaccgt gtgcatttat
     2941 tgggatagga aatagtgacc aagaaatgca gcagctaaac ttggaaggaa agaactattg
     3001 cacagccaaa acattgtata tatctgactc agacaagcga aagcacttca tgttgtctgt
     3061 aaagatgttc tatggcaaca gtgatgacat tggtgtgttc ctcagcaagc ggataaaagt
     3121 catctccaaa ccttccaaaa agaagcagtc attgaaaaat gctgacttat gcattgcctc
     3181 aggaacaaag gtggctctgt ttaatcgact acgatcccag acagttagta ccagatactt
     3241 gcatgtagaa ggaggtaatt ttcatgccag ttcacagcag tggggagcct tttttattca
     3301 tctcttggat gatgatgaat cagaaggaga agaattcaca gtccgagatg gctacatcca
     3361 ttatggacaa acagtcaaac ttgtgtgctc agttactggc atggcactcc caagattgat
     3421 aattaggaaa gttgataagc agaccgcatt attggatgca gatgatcctg tgtcacaact
     3481 ccataaatgt gcattttacc ttaaggatac agaaagaatg tatttgtgcc tttctcaaga
     3541 aagaataatt caatttcagg ccactccatg tccaaaagaa ccaaataaag agatgataaa
     3601 tgatggcgct tcctggacaa tcattagcac agataaggca gagtatacat tttatgaggg
     3661 aatgggccct gtccttgccc cagtcactcc tgtgcctgtg gtagagagcc ttcagttgaa
     3721 tggcggtggg gacgtagcaa tgcttgaact tacaggacag aatttcactc caaatttacg
     3781 agtgtggttt ggggatgtag aagctgaaac tatgtacagg tgtggagaga gtatgctctg
     3841 tgtcgtccca gacatttctg cattccgaga aggttggaga tgggtccggc aaccagtcca
     3901 ggttccagta actttggtcc gaaatgatgg aatcatttat tccaccagcc ttacctttac
     3961 ctacacacca gaaccagggc cgcggccaca ttgcagtgca gcaggagcaa tccttcgagc
     4021 caattcaagc caggtgcccc ctaacgaatc aaacacaaac agcgagggaa gttacacaaa
     4081 cgccagcaca aattcaacca gtgtcacatc atctacagcc acagtggtat cctaattgcc
     4141 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     4201 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata t
//
