LOCUS       sequence-163312-        4140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RELA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RELA_WT
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1540..1639)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1656..1675)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1657..1675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1680..1696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1680..1696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2127..2918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3011..3599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3500..3519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3753..3770
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3929..3956)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4048..4134)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggacgaa ctgttccccc tcatcttccc ggcagagcca gcccaggcct
      241 ctggccccta tgtggagatc attgagcagc ccaagcagcg gggcatgcgc ttccgctaca
      301 agtgcgaggg gcgctccgcg ggcagcatcc caggcgagag gagcacagat accaccaaga
      361 cccaccccac catcaagatc aatggctaca caggaccagg gacagtgcgc atctccctgg
      421 tcaccaagga ccctcctcac cggcctcacc cccacgagct tgtaggaaag gactgccggg
      481 atggcttcta tgaggctgag ctctgcccgg accgctgcat ccacagtttc cagaacctgg
      541 gaatccagtg tgtgaagaag cgggacctgg agcaggctat cagtcagcgc atccagacca
      601 acaacaaccc cttccaagtt cctatagaag agcagcgtgg ggactacgac ctgaatgctg
      661 tgcggctctg cttccaggtg acagtgcggg acccatcagg caggcccctc cgcctgccgc
      721 ctgtcctttc tcatcccatc tttgacaatc gtgcccccaa cactgccgag ctcaagatct
      781 gccgagtgaa ccgaaactct ggcagctgcc tcggtgggga tgagatcttc ctactgtgtg
      841 acaaggtgca gaaagaggac attgaggtgt atttcacggg accaggctgg gaggcccgag
      901 gctccttttc gcaagctgat gtgcaccgac aagtggccat tgtgttccgg acccctccct
      961 acgcagaccc cagcctgcag gctcctgtgc gtgtctccat gcagctgcgg cggccttccg
     1021 accgggagct cagtgagccc atggaattcc agtacctgcc agatacagac gatcgtcacc
     1081 ggattgagga gaaacgtaaa aggacatatg agaccttcaa gagcatcatg aagaagagtc
     1141 ctttcagcgg acccaccgac ccccggcctc cacctcgacg cattgctgtg ccttcccgca
     1201 gctcagcttc tgtccccaag ccagcacccc agccctatcc ctttacgtca tccctgagca
     1261 ccatcaacta tgatgagttt cccaccatgg tgtttccttc tgggcagatc agccaggcct
     1321 cggccttggc cccggcccct ccccaagtcc tgccccaggc tccagcccct gcccctgctc
     1381 cagccatggt atcagctctg gcccagaggc cccccgaccc agctcctgct ccactggggg
     1441 ccccggggct ccccaatggc ctcctttcag gagatgaaga cttctcctcc attgcggaca
     1501 tggacttctc agccctgctg agtcagatca gctcctaatt gccaactttc ttgtacaaag
     1561 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1621 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1681 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1741 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     1801 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     1861 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     1921 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     1981 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2041 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2101 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2161 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2221 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2281 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2341 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2401 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2461 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2521 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2581 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2641 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2701 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2761 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2821 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     2881 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     2941 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3061 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3721 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3781 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3841 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     3901 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     3961 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4021 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4081 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
//
