LOCUS       sequence-163316-        4371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RPS6KB1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RPS6KB1_WT
FEATURES             Location/Qualifiers
     source          1..4371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1771..1870)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1887..1906)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1888..1906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1911..1927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1911..1927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2358..3149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3242..3830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3731..3750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3984..4001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4160..4187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4279..4365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgaggcga cgaaggaggc gggacggctt ttacccagcc ccggacttcc
      241 gagacaggga agctgaggac atggcaggag tgtttgacat agacctggac cagccagagg
      301 acgcgggctc tgaggatgag ctggaggagg ggggtcagtt aaatgaaagc atggaccatg
      361 ggggagttgg accatatgaa cttggcatgg aacattgtga gaaatttgaa atctcagaaa
      421 ctagtgtgaa cagagggcca gaaaaaatca gaccagaatg ttttgagcta cttcgggtac
      481 ttggtaaagg gggctatgga aaggtttttc aagtacgaaa agtaacagga gcaaatactg
      541 ggaaaatatt tgccatgaag gtgcttaaaa aggcaatgat agtaagaaat gctaaagata
      601 cagctcatac aaaagcagaa cggaatattc tggaggaagt aaagcatccc ttcatcgtgg
      661 atttaattta tgcctttcag actggtggaa aactctacct catccttgag tatctcagtg
      721 gaggagaact atttatgcag ttagaaagag agggaatatt tatggaagac actgcctgct
      781 tttacttggc agaaatctcc atggctttgg ggcatttaca tcaaaagggg atcatctaca
      841 gagacctgaa gccggagaat atcatgctta atcaccaagg tcatgtgaaa ctaacagact
      901 ttggactatg caaagaatct attcatgatg gaacagtcac acacacattt tgtggaacaa
      961 tagaatacat ggcccatgaa atcttgatga gaagtggcca caatcgtgct gtggattggt
     1021 ggagtttggg agcattaatg tatgacatgc tgactggagc acccccattc actggggaga
     1081 atagaaagaa aacaattgac aaaatcctca aatgtaaact caatttgcct ccctacctca
     1141 cacaagaagc cagagatctg cttaaaaagc tgctgaaaag aaatgctgct tctcgtctgg
     1201 gagctggtcc tggggacgct ggagaagttc aagctcatcc attctttaga cacattaact
     1261 gggaagaact tctggctcga aaggtggagc ccccctttaa acctctgttg caatctgaag
     1321 aggatgtaag tcagtttgat tccaagttta cacgtcagac acctgtcgac agcccagatg
     1381 actcaactct cagtgaaagt gccaatcagg tctttctggg ttttacatat gtggctccat
     1441 ctgtacttga aagtgtgaaa gaaaagtttt cctttgaacc aaaaatccga tcacctcgaa
     1501 gatttattgg cagcccacga acacctgtca gcccagtcaa attttctcct ggggatttct
     1561 ggggaagagg tgcttcggcc agcacagcaa atcctcagac acctgtggaa tacccaatgg
     1621 aaacaagtgg catagagcag atggatgtga caatgagtgg ggaagcatcg gcaccacttc
     1681 caatacgaca gccgaactct gggccataca aaaaacaagc ttttcccatg atctccaaac
     1741 ggccagagca cctgcgtatg aatctatgat tgccaacttt cttgtacaaa gttggcatta
     1801 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1861 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1921 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1981 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2041 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2101 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2161 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2221 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2281 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2341 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2401 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2461 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2521 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2581 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2641 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2701 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2761 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2821 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2881 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2941 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3001 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3061 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3121 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3181 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4081 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4141 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4201 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4261 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4321 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
