LOCUS       sequence-163322-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLentiCRISPRv1-sgAAVS1
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..55
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             62..82
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caggtgtcgt gacgcgggat ccgccaccat ggattacaaa gacgatgacg ataagatggc
       61 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag
      121 catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      181 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      241 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
      301 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
      361 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
      421 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
      481 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
      541 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
      601 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
      661 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
      721 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
      781 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
      841 gggcctgacc cccaacttca agagcaactt cga
//
