LOCUS       sequence-163326-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLentiCRISPRv1-sgAAVS1
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..99)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gtcgccggcc tgcttcagca ggctgaagtt ggtggcgccg ctgccgctag ctttcttttt
       61 cttagcttga ccagctttct tagtagcagc aggacgcttg tcgcctccca gctgagacag
      121 gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      181 ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc
      241 aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat
      301 gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag
      361 gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg
      421 ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata
      481 gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt
      541 ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga
      601 gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag
      661 aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc
      721 tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca ccagcacaga
      781 ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt ccttctttct
      841 ggcgatcagc ttatcgctgt tcctcttggg caggatagac tctttgctga agccgcctgt
      901 ctgcacctcg gtctttttca cgatattcac tt
//
