LOCUS       sequence-163375-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting murine CD45.2 region 3. Co-expresses 
            SpCas9-2A-EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Kornete M, Marone R, Jeker LT
  TITLE     Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary
            T Cells.
  JOURNAL   J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 
            10.4049/jimmunol.1701121.
  PUBMED    29445007
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121."
COMMENT     Sequence Label: p206_LTJ_sgRNACD45.2_R3
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..124
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             131..151
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg ccaccatgga
       61 ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga
      121 taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
      181 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
      241 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      301 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      361 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      421 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      481 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
      541 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
      601 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
      661 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
      721 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
      781 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgg
//
