LOCUS       sequence-163376-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PHGDH
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaaacat catcatcatc
       61 atcaccagat ggcttttgca aatctgcgga aagtgctcat cagtgacagc ctggaccctt
      121 gctgccggaa gatcttgcaa gatggagggc tgcaggtggt ggaaaagcag aaccttagca
      181 aagaggagct gatagcggag ctgcaggact gtgaaggcct tattgttcgc tctgccacca
      241 aggtgaccgc tgatgtcatc aacgcagctg agaaactcca ggtggtgggc agggctggca
      301 caggtgtgga caatgtggat ctggaggccg caacaaggaa gggcatcttg gttatgaaca
      361 cccccaatgg gaacagcctc agtgccgcag aactcacttg tggaatgatc atgtgcctgg
      421 ccaggcagat tccccaggcg acggcttcga tgaaggacgg caaatgggag cggaagaagt
      481 tcatgggaac agagctgaat ggaaagaccc tgggaattct tggcctgggc aggattggga
      541 gagaggtagc tacccggatg cagtcctttg ggatgaagac tatagggtat gaccccatca
      601 tttccccaga ggtctcggcc tcctttggtg ttcagcagct gcccctggag gagatctggc
      661 ctctctgtga tttcatcact gtgcacactc ctctcctgcc ctccacgaca ggcttgctga
      721 atgacaacac ctttgcccag tgcaagaagg gggtgcgtgt ggtgaactgt gcccgtggag
      781 ggatcgtgga cgaaggcgcc ctgctccggg ccctgcagtc tggccagtgt gccggggctg
      841 cactggacgt gtttacggaa gagccgccac gggaccgggc cttgg
//
