LOCUS       sequence-163381-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PHGDH
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgggctttgt tagcagccgg atccttagaa gtggaactgg aaggcttcag tcacatgctg
       61 cttccacgct tccaggctgg gcagcaagga ggagatgccc atgacgtgcc aggtctcccc
      121 atctgacacc agtgaagtct ggtaggacag cagccgcacg cctgcctctg ccaggaggcc
      181 aatcatggta ggcagcattg cagggtcaga ggtctgagtc cggaatagga gcaggggcag
      241 gtccctgcgg agaggcactt ctggcctgaa gacagctcca ttgagcccct gcagtacagg
      301 tgtagtgcct tggaccaagc ccacagcctg gtaaggggcg cctgccaggg ccacggccag
      361 gaggcattcc ccgaagcctt gctcccctgg tgcagcaggg ctgtgggagg tggtgacatt
      421 gaggccagcc tctttcacca gcagcttagc gttcaccaag ttcacatccg cctgcttgga
      481 agcctctttc aggaggccga caatgactgc ggggcttagg cagttcccag cattcttcag
      541 ggatgttccc tgtgttatca cctggatggt ccctttgggg gacccagccc aggctcgcat
      601 cagtgtcccc agagcttctg ccagaccaat ccaaggcttg gtgtgtggag agaaggcact
      661 ggtaagggcc tgggcattca caacccccgt gagagatttc cccttcacca tgtccacgaa
      721 ctgaacagca atttcctccc cacagcggct ctgagcctcc ttggtgctgg cacccaggtg
      781 gggacagctg atgacattct catggtccac caaggcccgg tcccgtggcg gctcttccgt
      841 aaacacgtcc agtgcagccc cggcacactg gccagactgc agggcccgga gcagggcgcc
      901 ttcgtccacg atccctccac gggcacagtt caccacacgc acccccttct tgcactgggc
      961 aaaggtgttg tcattcagca agcctgtcgt gga
//
