LOCUS       sequence-163385-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PSAT1
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagaaataa ttttgtttaa actttaagaa ggagatatac atatgcatca tcatcatcat
       61 cacatggacg cccccaggca ggtggtcaac tttgggcctg gtcccgccaa gctgccgcac
      121 tcagtgttgt tagagataca aaaggaatta ttagactaca aaggagttgg cattagtgtt
      181 cttgaaatga gtcacaggtc atcagatttt gccaagatta ttaacaatac agagaatctt
      241 gtgcgggaat tgctagctgt tccagacaac tataaggtga tttttctgca aggaggtggg
      301 tgcggccagt tcagtgctgt ccccttaaac ctcattggct tgaaagcagg aaggtgtgcg
      361 gactatgtgg tgacaggagc ttggtcagct aaggccgcag aagaagccaa aaagtttggg
      421 actataaata tcgttcaccc taaacttggg agttatacaa aaattccaga tccaagcacc
      481 tggaacctca acccagatgc ctcctacgtg tattattgcg caaatgagac ggtgcatggt
      541 gtggagtttg actttatacc cgatgtcaag ggagcagtac tggtttgtga catgtcctca
      601 aacttcctgt ccaagccagt ggatgtttcc aagtttggtg tgatttttgc tggtgcccag
      661 aagaatgttg gctctgctgg ggtcaccgtg gtgattgtcc gtgatgacct gctggggttt
      721 gccctccgag agtgcccctc ggtcctggaa tacaaggtgc aggctggaaa cagctccttg
      781 tacaacacgc ctccatgttt cagcatctac gtcatgggct tggttctgga gtggattaaa
      841 aacaatggag gtgccgcggc catggagaag cttagctcca tcaaatctca aacaatttat
      901 gagattattg ataattctca aggattctac gtttgtccag tggagcccca aaatagaagc
      961 aagatgaata ttccattccg cattggcaat gccaaaggag atgatgcttt agaaaaaaga
     1021 tttctt
//
