LOCUS       sequence-163394-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP-Epn1 (18-157) Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub22-Epn1 (18-157) Dr
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             73..84
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             493..516
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gatagagatt gacatcccta tcagtgatag agatactgag cacatcagca ggacgcactg
       61 accgaattca ttaaagagga gaaaacgcgt atgagtaaag tagacatggt ttggatagtc
      121 ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccacctcag actctttgtg
      181 acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga tttggggaaa
      241 tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga aaaaggcatc
      301 aagtataagt ttgaagtcta cgagaagaaa gacaggcgcg ccggcggctc agcgggctcg
      361 ggaagcggag cgggatctgg cagcggcggt agcgcaggca gcagcgggtc aagcggtgcc
      421 tcgagcggca ggcgcgccct gcagatgggc agcagccatc accatcatca ccccatgggg
      481 atcgatacga ccctggaagt tctgttccag gggcccccgc ggatgtcaga ggcggagata
      541 aaggtaaggg aagcaacttc taatgatcca tggggtccct caagttctct gatgtcagag
      601 atcgctgacc tgacatacaa tgtagtggcc ttctcagaga ttatgagcat ggtctggaag
      661 agactcaacg accatgggaa aaactggaga catgtatata aagctatgac actgatggag
      721 tatctgataa agacgggttc agagcgggta gcacagcaat gcagggaaaa catctacgcc
      781 gttcaaacgc ttaaagactt ccagtacata gacagagacg gcaaagacca gggtgtgaat
      841 gtacgggaaa aggccaagca actggtgact ctgctaaaag acgaggagag gctacgagag
      901 gagaggatcc atgcactcaa gaccaaagag aagatggcac agacctgaac tagttaagt
//
