LOCUS       sequence-163396-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PSPH
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(813..831)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      827..874
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcccctctag aaataatttt gtttaaactt taagaaggag atatacatat gcatcatcat
       61 catcatcacg tgaattcgct cgagatggtc tcccactcag agctgaggaa gcttttctac
      121 tcagcagatg ctgtgtgttt tgatgttgac agcacggtca tcagagaaga aggaatcgat
      181 gagctagcca aaatctgtgg cgttgaggac gcggtgtcag aaatgacacg gcgagccatg
      241 ggcggggcag tgcctttcaa agctgctctc acagagcgct tagccctcat ccagccctcc
      301 agggagcagg tgcagagact catagcagag caacccccac acctgacccc cggcataagg
      361 gagctggtaa gtcgcctaca ggagcgaaat gttcaggttt tcctaatatc tggtggcttt
      421 aggagtattg tagagcatgt tgcttcaaag ctcaatatcc cagcaaccaa tgtatttgcc
      481 aataggctga aattctactt taacggtgaa tatgcaggtt ttgatgagac gcagccaaca
      541 gctgaatctg gtggaaaagg aaaagtgatt aaacttttaa aggaaaaatt tcattttaag
      601 aaaataatca tgattggaga tggtgccaca gatatggaag cctgtcctcc tgctgatgct
      661 ttcattggat ttggaggaaa tgtgatcagg caacaagtca aggataacgc caaatggtat
      721 atcactgatt ttgtagagct gctgggagaa ctggaagaat aaggatccgg ctgctaacaa
      781 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      841 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
      901 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      961 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
//
