LOCUS       sequence-163397-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-PSPH
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(721..738)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    787..811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(914..933)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctta ttcttccagt tctcccagca gctctacaaa
       61 atcagtgata taccatttgg cgttatcctt gacttgttgc ctgatcacat ttcctccaaa
      121 tccaatgaaa gcatcagcag gaggacaggc ttccatatct gtggcaccat ctccaatcat
      181 gattattttc ttaaaatgaa atttttcctt taaaagttta atcacttttc cttttccacc
      241 agattcagct gttggctgcg tctcatcaaa acctgcatat tcaccgttaa agtagaattt
      301 cagcctattg gcaaatacat tggttgctgg gatattgagc tttgaagcaa catgctctac
      361 aatactccta aagccaccag atattaggaa aacctgaaca tttcgctcct gtaggcgact
      421 taccagctcc cttatgccgg gggtcaggtg tgggggttgc tctgctatga gtctctgcac
      481 ctgctccctg gagggctgga tgagggctaa gcgctctgtg agagcagctt tgaaaggcac
      541 tgccccgccc atggctcgcc gtgtcatttc tgacaccgcg tcctcaacgc cacagatttt
      601 ggctagctca tcgattcctt cttctctgat gaccgtgctg tcaacatcaa aacacacagc
      661 atctgctgag tagaaaagct tcctcagctc tgagtgggag accatctcga gcgaattcac
      721 gtgatgatga tgatgatgca tatgtatatc tccttcttaa agtttaaaca aaattatttc
      781 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      841 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      901 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      961 gggctcatga gcgcttgttt cggcgt
//
