LOCUS       sequence-163399-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP-Epn1 (18-157) E42A Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub24-Epn1 (18-157) E42A Dr
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             494..517
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact
       61 gaccgaattc attaaagagg agaaaacgcg tatgagtaaa gtagacatgg tttggatagt
      121 cggaggcagt tctgtttacc aggaagccat gaatcaacca ggccacctca gactctttgt
      181 gacaaggatc atgcaggaat ttgaaagtga cacgtttttc ccagaaattg atttggggaa
      241 atataaactt ctcccagaat acccaggcgt cctctctgag gtccaggagg aaaaaggcat
      301 caagtataag tttgaagtct acgagaagaa agacaggcgc gccggcggct cagcgggctc
      361 gggaagcgga gcgggatctg gcagcggcgg tagcgcaggc agcagcgggt caagcggtgc
      421 ctcgagcggc aggcgcgccc tgcagatggg cagcagccat caccatcatc accccatggg
      481 gatcgatacg accctggaag ttctgttcca ggggcccccg cggatgtcag aggcggagat
      541 aaaggtaagg gaagcaactt ctaatgatcc atggggtccc tcaagttctc tgatgtcagc
      601 gatcgctgac ctgacataca atgtagtggc cttctcagag attatgagca tggtctggaa
      661 gagactcaac gaccatggga aaaactggag acatgtatat aaagctatga cactgatgga
      721 gtatctgata aagacgggtt cagagcgggt agcacagcaa tgcagggaaa acatctacgc
      781 cgttcaaacg cttaaagact tccagtacat agacagagac ggcaaagacc agggtgtgaa
      841 tgtacgggaa aaggccaagc aactggtgac tctgctaaaa gacgaggaga ggctacgaga
      901 ggagaggatc catgcactca agaccaaaga gaagatggca cagacctgaa ctagttaagt
      961 cgaacagaaa gtaatcgtat tgtacacgg
//
