LOCUS       sequence-163400-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP-Epn1 (18-157) E42A Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub24-Epn1 (18-157) E42A Dr
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(434..457)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             866..877
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttcaggtctg tgccatcttc tctttggtct tgagtgcatg gatcctctcc tctcgtagcc
       61 tctcctcgtc ttttagcaga gtcaccagtt gcttggcctt ttcccgtaca ttcacaccct
      121 ggtctttgcc gtctctgtct atgtactgga agtctttaag cgtttgaacg gcgtagatgt
      181 tttccctgca ttgctgtgct acccgctctg aacccgtctt tatcagatac tccatcagtg
      241 tcatagcttt atatacatgt ctccagtttt tcccatggtc gttgagtctc ttccagacca
      301 tgctcataat ctctgagaag gccactacat tgtatgtcag gtcagcgatc gctgacatca
      361 gagaacttga gggaccccat ggatcattag aagttgcttc ccttaccttt atctccgcct
      421 ctgacatccg cgggggcccc tggaacagaa cttccagggt cgtatcgatc cccatggggt
      481 gatgatggtg atggctgctg cccatctgca gggcgcgcct gccgctcgag gcaccgcttg
      541 acccgctgct gcctgcgcta ccgccgctgc cagatcccgc tccgcttccc gagcccgctg
      601 agccgccggc gcgcctgtct ttcttctcgt agacttcaaa cttatacttg atgccttttt
      661 cctcctggac ctcagagagg acgcctgggt attctgggag aagtttatat ttccccaaat
      721 caatttctgg gaaaaacgtg tcactttcaa attcctgcat gatccttgtc acaaagagtc
      781 tgaggtggcc tggttgattc atggcttcct ggtaaacaga actgcctccg actatccaaa
      841 ccatgtctac tttactcata cgcgttttct cctctttaat gaattcggtc agtgcgtcct
      901 gctgatgtgc tcagtatctc tatcactga
//
