LOCUS       sequence-163401-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-GAPDH
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcccctctag aaataatttt gtttaaactt taagaaggag atatacatat gcatcatcat
       61 catcatcacg tgaattcgct cgagatgggg aaggtgaagg tcggagtcaa cggatttggt
      121 cgtattgggc gcctggtcac cagggctgct tttaactctg gtaaagtgga tattgttgcc
      181 atcaatgacc ccttcattga cctcaactac atggtttaca tgttccaata tgattccacc
      241 catggcaaat tccatggcac cgtcaaggct gagaacggga agcttgtcat caatggaaat
      301 cccatcacca tcttccagga gcgagatccc tccaaaatca agtggggcga tgctggcgct
      361 gagtacgtcg tggagtccac tggcgtcttc accaccatgg agaaggctgg ggctcatttg
      421 caggggggag ccaaaagggt catcatctct gccccctctg ctgatgcccc catgttcgtc
      481 atgggtgtga accatgagaa gtatgacaac agcctcaaga tcatcagcaa tgcctcctgc
      541 accaccaact gcttagcacc cctggccaag gtcatccatg acaactttgg tatcgtggaa
      601 ggactcatga ccacagtcca tgccatcact gccacccaga agactgtgga tggcccctcc
      661 gggaaactgt ggcgtgatgg ccgcggggct ctccagaaca tcatccctgc ctctactggc
      721 gctgccaagg ctgtgggcaa ggtcatccct gagctgaacg ggaagctcac tggcatggcc
      781 ttccgtgtcc ccactgccaa cgtgtcagtg gtggacctga cctgccgtct agaaaaacct
      841 gccaaatatg atgacatcaa gaaggtggtg aagcaggcgt cggagggccc cctcaagggc
      901 atcctgggct acactgagca ccaggtggtc tcctctgact tcaacagcga cacccactcc
      961 tccacctttg acgctggggc tggcattgcc ctcaacgacc actttgtcaa gctcatttcc
     1021 tggtatgaca acgaatttgg
//
