LOCUS       sequence-163411-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pET30-2-GPD1
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcccctctag aaataatttt gtttaaactt taagaaggag atatacatat gaaacatcat
       61 catcatcatc accagatggc tagcaagaaa gtctgcattg taggctccgg gaactggggc
      121 tcagccatcg ccaagatcgt gggtggcaat gcagcccagc tggcacagtt tgacccacgg
      181 gtgaccatgt gggtatttga ggaagacatt ggaggcaaaa agctgactga gatcatcaac
      241 acgcagcatg agaatgtcaa atacctgcca gggcacaagt tgcccccaaa tgtggtggct
      301 gtcccagatg tggtccaggc tgcagaggat gctgacatcc tgatctttgt ggtgccccat
      361 cagttcatcg gcaagatctg tgaccagctc aagggccatc tgaaggcaaa cgccactggc
      421 atatctctta ttaagggggt agacgagggc cccaatgggc tgaagctcat ctcggaagtg
      481 attggggagc gcctcggcat ccccatgagt gtgctgatgg gggccaacat tgccagcgag
      541 gtggctgatg agaagttctg tgagacaacc attggctgca aggacccggc ccagggacaa
      601 ctcctgaaag agctgatgca gacaccaaac ttccgtatca cagtggtgca agaggtggac
      661 acagtagaga tctgtggagc cttaaagaat gtagtggccg tgggggctgg cttctgtgat
      721 ggcctgggct ttggcgacaa caccaaggcg gcagtgatcc ggctgggact catggagatg
      781 atagccttcg ccaagctctt ctgcagtggc cctgtgtcct ctgccacctt cttggagagc
      841 tgtggtgttg ctgacctgat cactacctgc tatggagggc ggaaccggaa agtggctgag
      901 gcctttgcgc gtacaggaaa gtccattgag cagctggaga aagagttgct gaatgggcag
      961 aaactgcagg ggcccgagac agcccgggag ctatacagca tcctccagca caagggcctg
     1021 gt
//
