LOCUS       sequence-163420-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle and expression vector. Replicates in E.coli and 
            Mycobacterium. Gives acetamide-inducible gene expression in 
            Mycobacterium. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  TITLE     Coleman Lab plasmids
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUS116
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     903..922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
       61 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
      121 ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaacttttg ctgagttgaa
      181 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
      241 aatcaccaac tggtccacct acaacaaagc tctcaccaac cgtggctccc tcactttctg
      301 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag
      361 acctcactag ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      541 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
      601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga ggggagaaag
      841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      961 cgatttttgt gatgctcgtc agggggg
//
