LOCUS       sequence-163425-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receives miRNA target sequences for testing using the dual 
            luciferase assay system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Moyle RL, Carvalhais LC, Pretorius LS, Nowak E, Subramaniam G, 
            Dalton-Morgan J, Schenk PM
  TITLE     An Optimized Transient Dual Luciferase Assay for Quantifying 
            MicroRNA Directed Repression of Targeted Sequences.
  JOURNAL   Front Plant Sci. 2017 Sep 20;8:1631. doi: 10.3389/fpls.2017.01631. 
            eCollection 2017.
  PUBMED    28979287
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-09-20"; volume: "8:1631. doi"; pages: " 
            10.3389/fpls.2017.01631. eCollection 2017"
COMMENT     Sequence Label: pGrDL_SPb
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..42
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
       61 caccgcggtg gcggccgcga tctggatttt agtactggat tttggtttta ggaattagaa
      121 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
      181 aacacatgag cgaaacccta taagaaccct aatttccctt atcgggaaac tactcacaca
      241 ttaactagtt caagagtaaa agatagtaaa accggtttgt actagctgga taccgatcct
      301 agggatagtg taatgaccgc atggcgactg cagggaagtc gacaaggaat tcttacacgg
      361 cgatctttcc gcccttcttg gcctttatga ggatctctct gatttttctt gcgtcgagtt
      421 ttccggtaag acctttcggt acttcgtcca caaacacaac tcctccgcgc aactttttcg
      481 cggttgttac ttgactggcg acgtaatcca cgatctcttt ttccgtcatc gtctttccgt
      541 gctccaaaac aacaacggcg gcgggaagtt caccggcgtc atcgtcggga agacctgcga
      601 cacctgcgtc gaagatgttg gggtgttgga gcaagatgga ttccaattca gcgggagcca
      661 cctgatagcc tttgtactta atcagagact tcaggcggtc aacgatgaag aagtgttcgt
      721 cttcgtccca gtaagctatg tctccagaat gtagccatcc atccttgtca atcaaggcgt
      781 tggtcgcttc cggattgttt acataaccgg acataatcat aggacctctc acacacagtt
      841 cgcctctttg attaacgccc agcgttttcc cggtatccag atccacaacc ttcgcttcaa
      901 aaaatggaac aactttaccg accgcgcccg gtttatcatc cccctcgggt gtaatcagaa
      961 tagctgatgt agtctcagtg agcccatatc cttgcctgat acctggcaga tggaacctct
     1021 tggcaaccgc ttccccgact tccttagaga ggggagcgcc accagaagca atttc
//
